Yeast Nitrogen Base without Amino acids and without para Amino Benzoic acid Size:1000g 5x is used in preparing

5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E

By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium

This buffer is used in 1D Gel Electrophoresis

Component g/l Peptone 5 Yeast Extract 0

Methionine and Trypthophan

Yeast Nitrogen Base without Amino acids and without para Amino Benzoic acid Size:1000g 5x is used in preparingPara Amino benzoic acid is omitted from this formulation of Yeast Nitrogen base w o Amino acids to provide the researcher the possibility to grow yeast cells in the absence of para Amino Benzoic acid or to replace this vitamin. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature